Burn Baby Burn

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Build 100 Inferno towers.

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From the Tower Encyclopedia: The Inferno tower can take out many enemies at once, but is fairly limited in its range. it is especially effective against swarmers or any aliens which clump together. its constant damage output on a target make it extremely useful against regenerating aliens. Because it is fire based, it is weaker against shields.

This achievement requires that you build 100 Inferno towers across all of your games. Your particular playing style may mean that this unlocks naturally without any particular effort. However, if you don't tend to use Inferno towers, or simply want it to unlock more promptly, you can play Chapter 19 in Practice mode on easy and play only Inferno towers. You can easily place 100 towers on this map and unlock the achievement at the end of the game, win or lose.